Protein, Peptide & Amino Acid Testing
HPLC-based amino acid profiling and protein content quantification for supplements and functional foods.
Protein and amino acid testing uses HPLC-based analysis to quantify total protein content, profile individual amino acids (including BCAAs), and detect protein spiking — the practice of adding low-cost amino acids to inflate nitrogen-based protein readings.
Accurate protein and amino acid testing is critical for sports nutrition, meal replacement, and protein supplement brands. Qalitex Laboratories provides HPLC-based amino acid analysis and protein quantification methods that go beyond the Kjeldahl/Dumas total nitrogen approach to deliver true protein and individual amino acid data.
Amino Acid Profile Testing
We provide complete amino acid profiling (AAA) using HPLC with pre-column derivatization, covering all essential and non-essential amino acids. This allows verification of label claims for BCAA content (leucine, isoleucine, valine) and total amino acid spectrum.
Protein Content Quantification
We use Kjeldahl, Dumas, and HPLC methods to determine total protein content, verifiable to label claims. For collagen and hydrolyzed proteins, we apply hydroxyproline-based methods to distinguish true protein from non-protein nitrogen sources.
Adulteration & Spiking Detection
Protein spiking — adding low-cost amino acids to inflate nitrogen readings — is a known industry problem. Our comprehensive amino acid profiling detects unusual enrichment of specific amino acids, protecting your brand and customers from adulterated raw materials.
Tests & Methods Included
- ✓ Complete amino acid profile (AAA)
- ✓ BCAA content verification
- ✓ Total protein quantification
- ✓ Hydroxyproline (collagen testing)
- ✓ Protein spiking detection
Regulatory Standards & Compliance
- USP <1057>
- AOAC 994.12 (amino acids)
- AOAC 990.03 (protein — Dumas)
- FDA cGMP 21 CFR Part 111
Industries We Serve
- Sports nutrition brands
- Protein supplement manufacturers
- Meal replacement products
- Collagen & peptide supplements
- Functional food brands
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Get a Quote →Frequently Asked Questions
What is amino acid profile testing?
Amino acid profile testing (AAA) uses HPLC with pre-column derivatization to identify and quantify all essential and non-essential amino acids in a product. This allows verification of BCAA content and total amino acid spectrum for sports nutrition and protein supplements.
What is protein spiking and how does Qalitex detect it?
Protein spiking is the practice of adding low-cost amino acids like glycine or taurine to inflate nitrogen-based protein readings. Qalitex detects spiking through comprehensive amino acid profiling that reveals unusual enrichment of specific amino acids.
How does Qalitex test collagen protein content?
For collagen and hydrolyzed proteins, Qalitex applies hydroxyproline-based methods that distinguish true collagen protein from non-protein nitrogen sources, providing accurate quantification beyond standard Kjeldahl or Dumas total nitrogen methods.
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